Curriculum Vitae

Ann M. Stock

Center for Advanced Biotechnology and Medicine
679 Hoes Lane
Piscataway, New Jersey 08854
phone: (732) 235-4844
fax: (732) 235-5289
stock@cabm.rutgers.edu

Education:

1979	A.B., Biochemistry
	University of California, Berkeley
1986	Ph.D., Comparative Biochemistry
	University of California, Berkeley

Research and Employment Experience:

1980-1986	Graduate Student - Laboratory of Professor Daniel E. Koshland, Jr., Department of Biochemistry, University of California, Berkeley
1986-1987	Postdoctoral Fellow - Laboratory of Professor Jeffry B. Stock, Molecular Biology Department, Princeton University, Princeton, NJ
1987-1989	Postdoctoral Fellow - Laboratory of Professor Clarence E. Schutt, Chemistry Department, Princeton University, Princeton, NJ
1989-1991	Postdoctoral Fellow - Laboratory of Professor Gregory A. Petsko, Rosenstiel Center, Brandeis University, Waltham, MA
1991-1997	Assistant Professor, Biochem. Dept., UMDNJ-Robert Wood Johnson Medical School
1991 -	Resident Faculty Member, Center for Advanced Biotechnology and Medicine
1994-1998	Assistant Investigator, Howard Hughes Medical Institute
1995 -	Member, Cancer Institute of New Jersey
1997-2001	Associate Professor, Biochemistry Dept., UMDNJ-Robert Wood Johnson Medical School
1998-2003	Associate Investigator, Howard Hughes Medical Institute
2001 -	Professor, Biochemistry Dept., UMDNJ-Robert Wood Johnson Medical School
2003 -	Investigator, Howard Hughes Medical Institute
2007 -	Associate Director, Center for Advanced Biotechnology and Medicine

Fellowships and Awards:

1977	University of California Alumni Scholarship
1979	Prytannean Society - service and scholarship
1979	Grace Fimonari Award to Biochemistry Dpt. senior
1979	Phi Beta Kappa
1981-1984	National Science Foundation Graduate Fellowship
1986	Theobald Smith Society (NJ division of ASM) Student Seminar Prize
1986-1987	National Cancer Institute Postdoctoral Training Grant
1987-1989	Damon Runyon-Walter Winchell Cancer Research Fund Postdoc. Fellowship
1989-1994	Lucille P. Markey Charitable Trust Biomedical Scholar Award
1992-1997	National Science Foundation Young Investigator Award
1993-1996	Sinsheimer Scholar Award
2002	Foundation of UMDNJ Excellence in Teaching Award
2002	Master Educator of UMDNJ
2003-2008	NIH NIGMS MERIT Award
2003	Molecular Biosciences Graduate Student Association Educator of the Year Award
2003	Cancer Institute of New Jersey Award of Hope
2004	New Jersey Association for Biomedical Research, Honoree
2006	Fellow, American Association for the Advancement of Science
2007	Fellow, American Academy of Microbiology

Publications:

(maiden name Maderis)

• Paoni, N.F., Maderis, A.M. and Koshland, D.E., Jr. (1981) Chemical sensing in bacteria. In Biochemistry of Taste and Olfaction. Academic Press, 459-474.
• Stock, J.B., Maderis, A.M. and Koshland, D.E., Jr. (1981) Bacterial chemotaxis in the absence of receptor carboxylmethylation. Cell 27: 37-44.
• Stock, A.M., Koshland, D.E., Jr. and Stock, J.B. (1985) Homologies between the Salmonella typhimurium CheY protein and proteins involved in the regulation of chemotaxis, membrane protein synthesis, and sporulation. Proc. Natl. Acad. Sci. U.S.A. 82: 7989-7993.
• Simms, S.A., Stock, A.M. and Stock, J.B. (1987) Purification and characterization of the S- adenosylmethionine:glutamyl methyltransferase which modifies membrane chemoreceptor proteins in bacteria. J. Biol. Chem. 262: 8537-8543.
• Stock, A., Schaeffer, E., Koshland, D.E., Jr. and Stock, J. (1987) A second type of protein methylation reaction in bacterial chemotaxis. J. Biol. Chem. 262: 8011-8014.
• Stock, A. and Stock, J. (1987) Purification and characterization of the CheZ protein of bacterial chemotaxis. J. Bacteriol. 169: 3301-3311.
• Borczuk, A., Stock, A. and Stock, J. (1987) S-adenosylmethionine may not be essential for signal transduction during bacterial chemotaxis. J. Bacteriol. 169: 3295-3300.
• Stock, A., Clarke, S., Clarke, C. and Stock, J. (1987) N-Terminal methylation of proteins -- structure, function, and specificity. FEBS Lett. 220: 8-14.
• Stock, J. and Stock, A. (1987) What is the role of receptor methylation in bacterial chemotaxis? Trends Biochem. Sci. 12: 371-375.
• Stock, A., Mottonen, J., Chen, T. and Stock, J. (1987) Identification of a possible nucleotide binding site in CheW, a protein required for sensory transduction in bacterial chemotaxis. J. Biol. Chem. 262: 535-537.
• Stock, A.M. (1988) N-Methylmethionine at the amino terminus of a protein required for bacterial chemotaxis. In Post-Translational Modification of Proteins and Ageing., V. Zappia, ed. Plenum Press, New York, 387-399.
• Stock, A., Chen, T., Welsh, D. and Stock, J. (1988) CheA, a central regulator of bacterial chemotaxis, belongs to a family of proteins that control gene expression in response to changing environmental conditions. Proc. Natl. Acad. Sci. U.S.A. 85: 1403-1407.
• Wylie, D., Stock, A., Wong, C.-Y. and Stock, J. (1988) Sensory transduction in bacterial chemotaxis involves phosphotransfer between Che proteins. Biochem. Biophys. Res. Commun. 151: 891-896.
• Stock, A.M., Wylie, D.C., Mottonen, J.M., Lupas, A.N., Ninfa, E.G., Ninfa, A.J., Schutt, C.E. and Stock, J.B. (1988) Phospho-proteins involved in bacterial signal transduction. Cold Spring Harbor Symp. Quant. Biol. 53: 49-57.
• Ninfa, A.J., Ninfa, E.G., Lupas, A.N., Stock, A., Magasanik, B. and Stock, J. (1988) Crosstalk between chemotaxis signal transduction proteins and the regulators of transcription of the Ntr regulon: evidence that nitrogen assimilation and chemotaxis are controlled by a common phosphotransfer mechanism. Proc. Natl. Acad. Sci. U.S.A. 85: 5492-5496.
• Stock, A.M., Mottonen, J.M., Stock, J.B. and Schutt, C.E. (1989) Three-dimensional structure of CheY, the response regulator of bacterial chemotaxis. Nature 337: 745-749.
• Sanders, D.A., Gillece-Castro, B.L., Stock, A.M., Burlingame, A.L. and Koshland, D.E., Jr. (1989) Identification of the site of phosphorylation of the chemotaxis response regulator protein, CheY. J. Biol. Chem. 264: 21770-21778.
• Stock, J.B., Ninfa, A.L. and Stock, A.M. (1989) Protein phosphorylation and the regulation of adaptive responses in bacteria. Microbiol. Rev. 53: 450-490.
• Stock, J.B., Stock, A.M. and Mottonen, J.M. (1990) Signal transduction in bacteria. Nature 344: 395-400.
• Lukat, G.S., Stock, A.M. and Stock, J.B. (1990) Divalent metal ion binding to CheY: its significance to phosphotransfer in bacterial chemotaxis. Biochemistry 29: 5436-5442.
• Krueger, J.K., Stock, A.M., Schutt, C.E. and Stock, J.B. (1990) Inclusion bodies from proteins produced at high levels in E. coli. In Protein folding: deciphering the second half of the genetic code., J.A. King and L.M. Geirasch, eds., AAAS, Washington, D.C. 136-142.
• Lukat, G.S., Lee, B., Mottonen, J.M., Stock, A.M. and Stock, J.B. (1991) Roles of the highly conserved aspartate and lysine residues in the response regulator of bacterial chemotaxis. J. Biol. Chem. 266: 8348-8354.
• Ninfa, E.G., Stock, A., Mowbray, S. and Stock, J. (1991) Reconstitution of the bacterial chemotaxis signal transduction system from purified components. J. Biol. Chem. 266: 9764-9770.
• Stock, J.B., Lukat, G.S. and Stock, A.M. (1991) Bacterial chemotaxis and the molecular logic of intracellular signal transduction networks. Ann. Rev. Biophys. Biophys. Chem. 20: 109-136.
• Lukat, G.S., McCleary, W.R., Stock, A.M. and Stock, J.B. (1992) Phosphorylation of bacterial response regulator proteins by low molecular weight phospho-donors. Proc. Natl. Acad. Sci. U.S.A. 89: 718-722.
• Rasmussen, B.F., Stock, A.M., Ringe, D. and Petsko, G.A. (1992) Crystalline ribonuclease A loses function below the dynamical transition at 220 K. Nature 357: 423-424.
• Stock, J., Surette, M., McCleary, W. and Stock, A. (1992) Signal transduction in bacterial chemotaxis. J. Biol. Chem. 267: 19753-19756.
• Singer, P.T., Carty, R.P., Berman, L.E., Schlichting, I., Stock, A., Smalas, A., Cai, Z., Mangel, W.F., Jones, K.W. and Sweet, R.M. (1992) Laue diffraction as a tool in dynamic studies: hydrolysis of a transiently stable intermediate in catalysis by trypsin. Phil. Trans. R. Soc. Lond. A340: 285-300.
• DeKoster, G.T., Robertson, A.D., Stock, A.M. and Stock, J.B. (1993) Urea and guanidine-HCl yield different unfolding free energies for CheY: Which denaturant provides the most reliable free energy values? Tech. in Protein Chem. 4: 533-540.
• Stock, A.M., Martinez-Hackert, E., Rasmussen, B.F., West, A., Stock, J.B., Ringe, D. and Petsko, G.A. (1993) Structure of the Mg²⁺-bound form of CheY and the mechanism of phosphoryl transfer in bacterial chemotaxis. Biochemistry 32: 13375-13380.
• tock, A.M. and Shinagawa, H. (1994) Signal transduction and phosphoproteins. In Molecular Biology of Phosphate in Microorganisms., A.M. Torriani, S. Silver and E. Yagil, eds., American Society for Microbiology, Washington, D.C. 282-284.
• West, A.H., Martinez-Hackert, E. and Stock, A.M. (1994) Structural basis for the mechanism of phosphoryl transfer in bacterial chemotaxis. In Molecular Biology of Phosphate in Microorganisms., A.M. Torriani, S. Silver and E. Yagil, eds., American Society for Microbiology, Washington, D.C. 309-314.
• West, A.H., Djordjevic, S., Martinez-Hackert, E. and Stock, A.M. (1995) Purification, crystallization and preliminary X-ray diffraction analyses of the bacterial chemotaxis receptor modifying enzymes. Proteins: Struct. Funct. Genet. 21: 345-350.
• West, A.H., Martinez-Hackert, E. and Stock, A.M. (1995) Crystal structure of the catalytic domain of the chemotaxis receptor methylesterase, CheB. J. Mol. Biol. 250: 276-290.
• Stock, A.M. and Mowbray, S.L. (1995) Bacterial chemotaxis: a field in motion. Curr. Opinion Struct. Biol. 5: 744-751.
• Martinez-Hackert, E., Harlocker, S., Inouye, M., Berman, H.M. and Stock, A.M. (1996) Crystallization, X-ray studies and site-directed cysteine mutagenesis of the DNA-binding domain of OmpR. Protein Sci. 5: 1429-1433.
• Lee, P.-J. and Stock, A.M. (1996) Characterization of the genes and proteins of a two- component system from the hyperthermophilic bacterium Thermotoga maritima. J. Bacteriol. 178: 5579-5585.
• Martinez-Hackert, E. and Stock, A.M. (1997) The DNA-binding domain of OmpR: crystal structure of a winged helix transcription factor. Structure 5: 109-124.
• Djordjevic, S. and Stock, A.M. (1997). Crystal structure of the chemotaxis receptor methyltransferase CheR suggests a conserved structural motif for binding S-adenosylmethionine. Structure 5: 545-558.
• Martinez-Hackert,E. and Stock, A.M. (1997) Structural relationships in the OmpR family of winged-helix transcription factors. J. Mol. Biol. 269: 301-312.
• Stock, A.M. (1997) Energy sensors for aerotaxis in Escherichia coli: something old, something new. Proc. Natl. Acad. Sci. USA 94: 10487-10489.
• Djordjevic, S., Goudreau, P., Xu, Q., Stock, A.M. and West, A.H. (1998) Structural basis for methylesterase CheB regulation by a phosphorylation- activated domain. Proc. Natl. Acad. Sci. USA 95: 1381-1386.
• Goudreau, P.N. and Stock, A.M. (1998) Signal transduction in bacteria: molecular mechanisms of stimulus-response coupling. Curr. Opin. Microbiol. 1: 160-169.
• Djordjevic, S. and Stock, A.M. (1998) Chemotaxis receptor recognition by protein methyltransferase CheR. Nature Struct. Biol. 5: 446-450.
• Anand, G.S., Goudreau, P.N. and Stock, A.M. (1998) Activation of methylesterase CheB: evidence of a dual role for the regulatory domain. Biochemistry 37: 14038-14047.
• Goudreau, P.N., Lee, P.-J. and Stock, A.M. (1998) Stabilization of the phospho-aspartyl residue involved in a two-component signal transduction system in Thermotoga maritima. Biochemistry 37: 14575-14584.
• Djordjevic S. and Stock, A.M. (1998) Structural analysis of bacterial chemotaxis proteins: components of a dynamic signaling system. J. Struct. Biol. 124: 189-200.
• Harrison-McMonagle, P., Denissova, N., Martinez-Hackert, E., Ebright, R.H. and Stock, A.M. (1999) Orientation of OmpR monomers within an OmpR:DNA complex determined by DNA affinity cleaving. J. Mol. Biol. 285: 555-566.
• Robinson, V.L. and Stock, A.M. (1999) High energy exchange: proteins that make or break phosphoramidate bonds. Structure 7: R47-R53.
• Zhang, Z., Komives, E.A., Sugio, S., Blackow, S.C., Narayana, N., Xuong, N.H., Stock, A.M., Petsko, G.A. and Ringe, D. (1999) The role of water in the catalytic efficiency of triosephosphate isomerase. Biochemistry 38: 4389-4397.
• Stock, A.(1999) Relating dynamics to function. Nature 400: 221-222.
• Djordjevic, S., Stock, A.M., Chen, Y. and Stock, J. (1999) Protein methyltransferases involved in signal transduction. In Structure and Function of AdoMet-Dependent Methyltransferases. X. Cheng and R.M. Blumenthal, eds., World Scientific Publishing Co. /Imperial College Press 149-183.
• Stock, A.(1999) A nonlinear stimulus-response relation in bacterial chemotaxis. Proc. Natl. Acad. Sci. U.S.A. 96: 10945-10947.
• Schlichting, I., Berendzen, J., Chu, K., Stock, A.M., Maves, S.A., Benson, D.E., Sweet, R.M., Ringe, D., Petsko, G.A. and Sligar, S.G. (2000) The catalytic pathway of cytochrome P450cam at atomic resolution. Science 287: 1615-1622.
• Stock, A.M., Robinson, V.L. and Goudreau, P.N. (2000) Two-component signal transduction. Annu. Rev. Biochem. 69: 183-215.
• Buckler, D.R., Anand, G.S. and Stock, A.M. (2000) Response regulator phosphorylation and activation: a two way street? Trends Microbiol. 8: 153-155.
• Anand, G.S., Goudreau, P.N., Lewis, J.K. and Stock, A.M. (2000) Evidence for phosphorylation-dependent conformational changes in methylesterase CheB. Protein Sci. 9: 898-906.
• Buckler, D.R. and Stock, A.M. (2000) Synthesis of [³²P]phosphoramidate for use as a low molecular weight phosphodonor reagent. Anal. Biochem. 283: 222-227.
• Robinson, V.L., Buckler,D.R. and Stock, A.M. (2000) A tale of two components: a novel kinase and a regulatory switch. Nature Struct. Biol. 7: 626-633.
• Hughes, C.A., Mandell, J.G., Anand, G.S., Stock, A.M. and Komives, E.A. (2001) Phosphorylation causes subtle changes in solvent accessibility at the interdomain interface of methylesterase CheB. J. Mol. Biol. 307: 967-976.
• West, A.H. and Stock, A.M. (2001) Histidine kinases and response regulator proteins in two-component signaling systems. Trends Biochem. Sci. 26: 369-376.
• Hughes, S.H. and Stock, A.M. (2001) Techniques in molecular biology and protein expression. In International Tables for Macromolecular Crystallography Vol. F, E. Arnold and M.G. Rossmann, eds., International Union of Crystallography and Kluwer Academic Publishers, Dordrecht/Boston/London pp. 65-80.
• Saxl, R.L., Anand, G.S., and Stock, A.M. (2001) Synthesis and biochemical characterization of a phosphorylated analog of the response regulator CheB. Biochemistry 40: 12896-12903.
• Bourret, R.B., Charon, N.W., Stock, A.M. and West, A.H. (2002) Bright lights, abundant operons: fluorescence and genomic technologies advance studies of bacterial locomotion and signal transduction: Review of the BLAST meeting, Cuernavaca, Mexico, 14 to 19 January 2001. J. Bacteriol. 184: 1-17.
• Buckler, D.R., Zhou, Y. and Stock, A.M. (2002) Evidence of intradomain and interdomain flexibility in an OmpR/PhoB homolog from Thermotoga maritima. Structure 10: 153-164.
• Bourret, R.B. and Stock, A.M. (2002) Molecular information processing: lessons from bacterial chemotaxis. J. Biol. Chem. 277: 9625-9628.
• Anand, G.S. and Stock, A.M. (2002) The kinetic basis for the stimulatory effect of phosphorylation on the methylesterase activity of CheB. Biochemistry 41: 6752-6760.
• Robinson, V.L., Hwang, J., Fox, E., Inouye, M. and Stock, A.M. (2002) Domain arrangement of Der, a switch protein containing two GTPase domains. Structure 10: 1649-1658.
• Stock, A.M. and West, A.H. (2003) Response regulator proteins and their interactions with histidine protein kinases. In Histidine Kinases in Signal Transduction M. Inouye and R. Dutta, eds., Academic Press, New York, New York. 237-271.
• Friedland, N., Liou, H.-L., Lobel, P. and Stock, A.M. (2003) Structure of a cholesterol-binding protein efficient in Niemann-Pick type C2 disease. Proc. Natl. Acad. Sci. USA 100: 2512-2517.
• Robinson, V.L., Wu, T. and Stock, A.M. (2003) Structural analysis of the domain interface in DrrB, a response regulator of the OmpR/PhoB subfamily. J. Bacteriol. 185: 4186-4194.
• Perez, E., West, A.H., Stock, A.M. and Djordjevic, S. (2004) Discrimination between different methylation states of chemotaxis receptor Tar by receptor methyltransferase CheR. Biochemistry 45: 953-961.
• Lubetsky, J.B. and Stock, A.M. (2005) Two-component signal transduction and chemotaxis. In Structural Biology of Bacterial Pathogens G. Waksman, M. Caparon and S. Hultgren, eds., American Society for Microbiology Press, Washington, D.C. 17-36.
• Toro- Roman, A., Mack, T.R. and Stock, A.M. (2005) Structural analysis and solution studies of the activated regulatory domain of the response regulator ArcA: a symmetric dimer mediated by the alpha4-beta5-alpha5 face. J. Mol. Biol. 349: 11-26.
• Bachhawat, P., Swapna, G.V., Montelione, G.T. and Stock, A.M. (2005) Mechanism of activation for transcription factor PhoB suggested by different modes of dimerization in the inactive and active states. Structure 13: 1353-1363.
• Toro- Roman, A., Wu, T. and Stock, A.M. (2005) A common dimerization interface in bacterial response regulators KdpE and TorR. Prot. Sci. 14: 3077- 3088.
• Perez, E., Zheng, H. and Stock, A.M. (2006) Identification of methylation sites in Thermotoga maritima chemotaxis receptors. J. Bacteriol. 188: 4093-4100.
• Guhaniyogi, J., Robinson, V.L. and Stock, A.M. (2006) Crystal structures of beryllium fluoride-free and beryllium fluoride-bound CheY in complex with the conserved C-terminal peptide of CheZ reveal dual binding modes specific to CheY conformation. J. Mol. Biol. 359: 624-645.
• Liou, H.-L., Dixit, S.S., Xu, S., Tint, G.S., Stock, A.M. and Lobel, P. (2006) NPC2, the protein deficient in Niemann-Pick C2 disease, consists of multiple glycoforms that bind a variety of sterols. J. Biol. Chem. 281: 36710-36723.
• Stock, A.M. (2006) Transmembrane signaling by asymmetry. Nat. Struct. Mol. Biol. 13: 861- 862.
• Stock, A.M. and Guhaniyogi, J. (2006) A new perspective on response regulator activation. J. Bacteriol. 188: 7328-7330.
• Perez, E. and Stock, A.M. (2007) Characterization of the Thermotoga maritima chemotaxis methylation system that lacks methyltransferase CheR:MCP tethering. Mol. Microbiol. 63: 363-378.
• Gao, R., Mack, T.R. and Stock, A.M. (2007) Bacterial response regulators: versatile regulatory strategies from common domains. Trends Biochem. Sci. 32: 225- 234.
• Friedland, N., Mack, T.R., Yu, M., Hung, L.-W., Terwilliger, T.C., Waldo, G.S. and Stock, A.M. (2007) Domain orientation in the inactive response regulator Mycobacterium tuberculosis MtrA provides a barrier to activation. Biochemistry 46: 6733- 6743.
• Bachhawat, P. and Stock, A.M. (2007) Crystal structures of the receiver domain of response regulator PhoP from Escherichia coli in the absence and presence of the phosphoryl analog beryllofluoride. J. Bacteriol. 189: 5987-5995.
• Dixit, S.S., Sleat, D.E., Stock, A.M. and Lobel, P. (2007) Do mammalian NPC1 and NPC2 play a role in intestinal cholesterol absorption? Biochem. J. 408: 1-5.
• Guhaniyogi, J., Wu, T., Patel, S.S. and Stock, A.M. (2008) Interaction of CheY with the C- terminal peptide of CheZ. J. Bacteriol. 190: 1419-1428.
• Xu, S., Benoff, B., Liou, H.-L., Lobel, P. and Stock, A.M. (2007) Structural basis of sterol binding by NPC2, a lysosomal protein deficient in Niemann-Pick type C2 disease. J. Biol. Chem. 282: 23525-23531.
• Barbieri, C.M. and Stock, A.M. (2008) Universally applicable methods for both in vitro and in vivo monitoring of response regulator aspartate phosphorylation using Phos- tag^TM based reagents. Anal. Biochem. 376: 73-82.
• Xu, S., Sidote, D.J., Barbieri, C.M., Wu, T. and Stock, A.M. (2008) Structure of the Staphylococcus aureus AgrA LytTR domain bound to DNA reveals a beta fold with an unusual mode of binding. Structure 16: 727-735.